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polypropylene tubes  (Greiner Bio)


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    Structured Review

    Greiner Bio polypropylene tubes
    Polypropylene Tubes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/conical+bottom/Tube+50+Ml+Pp+30%2F115+Mm+Conical+Bottom/bio_rxiv__64898__2026__03__27__714766-48-14-16
    Average 96 stars, based on 254 article reviews
    polypropylene tubes - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Novel markers for high-throughput protoplast-based analyses of phytohormone signaling
    Article Snippet: .. Protoplast transfection was performed in 96-well plates with a conical bottom (Greiner BioOne 651261). ..

    other:

    Article Title: BEHAV3D: a 3D live imaging platform for comprehensive analysis of engineered T cell behavior and tumor response.
    Article Snippet: Modeling immuno-oncology by using patient-derived material and immune cell co-cultures can advance our understanding of immune cell tumor targeting in a patient-specific manner, offering leads to improve cellular immunotherapy.. However, fully exploiting these living cultures requires analysis of the dynamic cellular features modeled, for which protocols are currently limited.. Here, we describe the application of BEHAV3D, a platform that implements multi-color live 3D imaging and computational tools for: (i) analyzing tumor death dynamics at both single-organoid or cell and population levels, (ii) classifying T cell behavior and (iii) producing data-informed 3D images and videos for visual inspection and further insight into obtained results.

    Article Title: Mapping T cell dynamics to molecular profiles through behavior-guided transcriptomics.
    Article Snippet: The rise of cellular immunotherapy for cancer treatment has led to the utilization of immune oncology cocultures to simulate T cell interactions with cancer cells for assessing their antitumor response.. Previously, we developed BEHAV3D, a three-dimensional live imaging platform of patient-derived tumor organoid (PDO) and engineered T cell cocultures, that analyzes T cells’ dynamics to gain crucial insights into their behavior during tumor targeting.. However, live imaging alone cannot determine the molecular drivers behind these behaviors.

    Hood:

    Article Title: Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids
    Article Snippet: .. ● 1.5-ml Safe-lock tubes (Eppendorf, cat. no. 0030120086) ● 2.0-ml Safe-lock tubes (Eppendorf, cat. no. 0030120094) ● 2.0-ml Cryovials (Greiner Bio-One, cat. no. 122263) ● Biosafety cabinet ● −80 °C freezer (e.g., Eppendorf CryoCube F740hi, cat. no. 740320011) ● CoolCell LX Freezing Container (Corning, cat. no. CLS432001) ● Counting chamber (e.g., Bürker-Türk, Merck, cat. no. BR719520) ● Micro-dissecting forceps (Merck, cat. no. F3767) ● Cell culture dishes, 60 mm × 15 mm (Greiner Bio-One, cat. no. 664160) ● Cell strainer, 70 μm (Greiner Bio-One, cat. no. 542070) ● Cell strainer, 100 μm (Greiner, cat. no. 542000) ● Centrifuge for 15/50 ml Falcon Bio-One tubes (e.g., Eppendorf, cat. no. 5804R) ● CO2 incubator, 37 °C and 5% CO2 (e.g., Thermo Fisher Scientific, model no. 4141) ● Disposable scalpels (Swann-Morton, code 0501) ● Electroporation chambers (2 mm), Cuvettes Plus (Thermo Fisher Scientific, cat. no. BTX620) ● Falcon tubes, 15 ml, conical bottom (Greiner Bio-One, cat. no. 188271) ● Falcon tubes, 50 ml, conical bottom (Greiner Bio-One, cat. nos. .. 227261) ● Gene electroporator (NEPA GENE, model no. NEPA21 Super Electroporator) ● Heated incubator oven (e.g., Panasonic, model no. MIR-H163-PE) ● Petri dish, glass, 100 mm × 20 mm (VMR, cat. no. 391-0579) ● Inverted bright-field microscope, objectives ×5, ×10 and ×20 (e.g., Leica, model no. DMi1) ● Microcentrifuge (e.g., Eppendorf, cat. no. 5424R) ● Millex-GS Syringe Filter Unit, 0.22 μm (Merck, cat. no. SLGS033SS) ● Multi-well suspension plates, 12-, 24- and 48-well (Greiner Bio-One, cat. nos.

    Cell Culture:

    Article Title: Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids
    Article Snippet: .. ● 1.5-ml Safe-lock tubes (Eppendorf, cat. no. 0030120086) ● 2.0-ml Safe-lock tubes (Eppendorf, cat. no. 0030120094) ● 2.0-ml Cryovials (Greiner Bio-One, cat. no. 122263) ● Biosafety cabinet ● −80 °C freezer (e.g., Eppendorf CryoCube F740hi, cat. no. 740320011) ● CoolCell LX Freezing Container (Corning, cat. no. CLS432001) ● Counting chamber (e.g., Bürker-Türk, Merck, cat. no. BR719520) ● Micro-dissecting forceps (Merck, cat. no. F3767) ● Cell culture dishes, 60 mm × 15 mm (Greiner Bio-One, cat. no. 664160) ● Cell strainer, 70 μm (Greiner Bio-One, cat. no. 542070) ● Cell strainer, 100 μm (Greiner, cat. no. 542000) ● Centrifuge for 15/50 ml Falcon Bio-One tubes (e.g., Eppendorf, cat. no. 5804R) ● CO2 incubator, 37 °C and 5% CO2 (e.g., Thermo Fisher Scientific, model no. 4141) ● Disposable scalpels (Swann-Morton, code 0501) ● Electroporation chambers (2 mm), Cuvettes Plus (Thermo Fisher Scientific, cat. no. BTX620) ● Falcon tubes, 15 ml, conical bottom (Greiner Bio-One, cat. no. 188271) ● Falcon tubes, 50 ml, conical bottom (Greiner Bio-One, cat. nos. .. 227261) ● Gene electroporator (NEPA GENE, model no. NEPA21 Super Electroporator) ● Heated incubator oven (e.g., Panasonic, model no. MIR-H163-PE) ● Petri dish, glass, 100 mm × 20 mm (VMR, cat. no. 391-0579) ● Inverted bright-field microscope, objectives ×5, ×10 and ×20 (e.g., Leica, model no. DMi1) ● Microcentrifuge (e.g., Eppendorf, cat. no. 5424R) ● Millex-GS Syringe Filter Unit, 0.22 μm (Merck, cat. no. SLGS033SS) ● Multi-well suspension plates, 12-, 24- and 48-well (Greiner Bio-One, cat. nos.

    Article Title: Genetic in vivo engineering of human T lymphocytes in mouse models.
    Article Snippet: Receptor targeting of vector particles is a key technology to enable cell type–specific in vivo gene delivery.. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them to express chimeric antigen receptors (CARs).. Here, we provide detailed protocols for the generation of CD4and CD8-targeted LVs (which takes ~9 d in total).

    Electroporation:

    Article Title: Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids
    Article Snippet: .. ● 1.5-ml Safe-lock tubes (Eppendorf, cat. no. 0030120086) ● 2.0-ml Safe-lock tubes (Eppendorf, cat. no. 0030120094) ● 2.0-ml Cryovials (Greiner Bio-One, cat. no. 122263) ● Biosafety cabinet ● −80 °C freezer (e.g., Eppendorf CryoCube F740hi, cat. no. 740320011) ● CoolCell LX Freezing Container (Corning, cat. no. CLS432001) ● Counting chamber (e.g., Bürker-Türk, Merck, cat. no. BR719520) ● Micro-dissecting forceps (Merck, cat. no. F3767) ● Cell culture dishes, 60 mm × 15 mm (Greiner Bio-One, cat. no. 664160) ● Cell strainer, 70 μm (Greiner Bio-One, cat. no. 542070) ● Cell strainer, 100 μm (Greiner, cat. no. 542000) ● Centrifuge for 15/50 ml Falcon Bio-One tubes (e.g., Eppendorf, cat. no. 5804R) ● CO2 incubator, 37 °C and 5% CO2 (e.g., Thermo Fisher Scientific, model no. 4141) ● Disposable scalpels (Swann-Morton, code 0501) ● Electroporation chambers (2 mm), Cuvettes Plus (Thermo Fisher Scientific, cat. no. BTX620) ● Falcon tubes, 15 ml, conical bottom (Greiner Bio-One, cat. no. 188271) ● Falcon tubes, 50 ml, conical bottom (Greiner Bio-One, cat. nos. .. 227261) ● Gene electroporator (NEPA GENE, model no. NEPA21 Super Electroporator) ● Heated incubator oven (e.g., Panasonic, model no. MIR-H163-PE) ● Petri dish, glass, 100 mm × 20 mm (VMR, cat. no. 391-0579) ● Inverted bright-field microscope, objectives ×5, ×10 and ×20 (e.g., Leica, model no. DMi1) ● Microcentrifuge (e.g., Eppendorf, cat. no. 5424R) ● Millex-GS Syringe Filter Unit, 0.22 μm (Merck, cat. no. SLGS033SS) ● Multi-well suspension plates, 12-, 24- and 48-well (Greiner Bio-One, cat. nos.

    Concentration Assay:

    Article Title: A high-throughput assay for the measurement of Ca 2+ -oscillations and insulin release from uniformly sized β-cell spheroids
    Article Snippet: .. Test compounds were prepared to the correct concentration and aliquoted into a clear 96-well plate with conical bottom (651101, Greiner Bio-One International GmbH, Austria) in accordance with the protocol for the μCELL FDSS fluorescence plate imager. .. All test compounds were of analytical standard or certified reference material purity (>96%) and obtained from Sigma-Aldrich (St. Louis, Mo).

    Fluorescence:

    Article Title: A high-throughput assay for the measurement of Ca 2+ -oscillations and insulin release from uniformly sized β-cell spheroids
    Article Snippet: .. Test compounds were prepared to the correct concentration and aliquoted into a clear 96-well plate with conical bottom (651101, Greiner Bio-One International GmbH, Austria) in accordance with the protocol for the μCELL FDSS fluorescence plate imager. .. All test compounds were of analytical standard or certified reference material purity (>96%) and obtained from Sigma-Aldrich (St. Louis, Mo).

    Sterility:

    Article Title: Genetic in vivo engineering of human T lymphocytes in mouse models.
    Article Snippet: Receptor targeting of vector particles is a key technology to enable cell type–specific in vivo gene delivery.. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them to express chimeric antigen receptors (CARs).. Here, we provide detailed protocols for the generation of CD4and CD8-targeted LVs (which takes ~9 d in total).

    Membrane:

    Article Title: Genetic in vivo engineering of human T lymphocytes in mouse models.
    Article Snippet: Receptor targeting of vector particles is a key technology to enable cell type–specific in vivo gene delivery.. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them to express chimeric antigen receptors (CARs).. Here, we provide detailed protocols for the generation of CD4and CD8-targeted LVs (which takes ~9 d in total).

    Filtration:

    Article Title: Genetic in vivo engineering of human T lymphocytes in mouse models.
    Article Snippet: Receptor targeting of vector particles is a key technology to enable cell type–specific in vivo gene delivery.. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them to express chimeric antigen receptors (CARs).. Here, we provide detailed protocols for the generation of CD4and CD8-targeted LVs (which takes ~9 d in total).

    Pore Size:

    Article Title: Genetic in vivo engineering of human T lymphocytes in mouse models.
    Article Snippet: Receptor targeting of vector particles is a key technology to enable cell type–specific in vivo gene delivery.. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them to express chimeric antigen receptors (CARs).. Here, we provide detailed protocols for the generation of CD4and CD8-targeted LVs (which takes ~9 d in total).

    Transferring:

    Article Title: Genetic in vivo engineering of human T lymphocytes in mouse models.
    Article Snippet: Receptor targeting of vector particles is a key technology to enable cell type–specific in vivo gene delivery.. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them to express chimeric antigen receptors (CARs).. Here, we provide detailed protocols for the generation of CD4and CD8-targeted LVs (which takes ~9 d in total).



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